Scientific Publications

Mikio Nishizawa, et al.

Anti-inflammatory Effects of Enzyme-treated Asparagus Extract and Its Constituents in Hepatocytes

FFHD. 2016; 6(2): 91-109.

Tomohiro Ito, et al.

Enzyme-Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

J Food Sci. 2014; 79(3): H413-9.

Jun Takanari, et al.

Effect of Enzyme-Treated Asparagus Extract (ETAS) on Psychological Stress in Healthy Individuals

J Nutr Sci Vitaminol. 2016; 62(3): 198-205.

Tomohiro Ito, et al.

Effects of Enzyme-Treated Asparagus Extract on Heat Shock Protein 70, Stress Indices, and Sleep in Healthy Adult Men

J Nutr Sci Vitaminol. 2014; 60(4): 283-90.

Tomohiro Ito, et al.

Enzyme-Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

J Food Sci. 2014; 79(3): H413-9.

Tsukasa Takahashi, et al.

An extract from asparagus improves sleep quality and reduces sleepiness and fatigue on awakening: A pilot randomized controlled trial

FFHD. 2025; 15(4): 191-204.

Saki Sakai, et al.

Improved Sleep Quality and Work Performance Among Shift Workers Consuming a “Foods with Function Claims” Containing Asparagus Extract

J UOEH. 2021; 43(1): 15-23.

Naoyuki Deguchi, et al.

Effects of food containing asparagus extract on sleep state, fatigue and safe driving in taxi drivers: An open-label, randomized, parallel-group comparison study

Journal of the Japanese Council of Traffic Science. 2019; 19(1): 33-44.

Manami Kato, et al.

Effects of Food Containing Asparagus Extract (ONR‒8) on Sleep Conditions in Evening‒type Individuals -A Randomized, Double‒blind, Placebo‒controlled, Crossover, Exploratory Study-

Japanese Pharmacology & Therapeutics. 2016; 44(6): 887-893.

Manami Kato, et al.

Effects of Food Containing Asparagus Extract (ONR‒8) on Alertness upon Awakening and Sleep Quality on Monday in Healthy Participants with a Discrepancy between the Sleep‒wake Rhythm on Workdays and Weekends -A Randomized, Double‒blind, Placebo‒controlled, Crossover Study-

Japanese Pharmacology Therapeutics. 2016; 44(5): 743-750.

Tomohiro Ito, et al.

Effects of Enzyme-Treated Asparagus Extract on Heat Shock Protein 70, Stress Indices, and Sleep in Healthy Adult Men

J Nutr Sci Vitaminol. 2014; 60(4): 283-90.

Natalia Mikhailichenko, et al.

Evaluation of the beneficial effects of ETAS® on normal aging or mild cognitive impairment subjects: A pilot randomized controlled trial

Journal of Pharmacy & Pharmacognosy Research, 12 (1), 154-165, 2024

Takayuki Zenimoto, et al.

Effect of a Standardized Extract of Asparagus officinalis Stem (ETAS®50) on Cognitive Function, Psychological Symptoms, and Behavior in Patients with Dementia: A Randomized Crossover Trial

Evid Based Complement Alternat Med. 2023; 2023: 9960094.

Yin-Ching Chan, et al.

A Standardized Extract of Asparagus officinalis Stem (ETAS®) Ameliorates Cognitive Impairment, Inhibits Amyloid Deposition via BACE-1 and Normalizes Circadian Rhythm Signaling via MT1 and MT2

Nutrients. 2019; 11(7): 1631.

Junetsu Ogasawara, et al.

ETAS, an Enzyme-treated Asparagus Extract, Attenuates Amyloid β-Induced Cellular Disorder in PC12 Cells

Nat Prod Commun. 2014; 9(4): 561-4.

Zhanglong Peng, et al.

Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein

J Immunol Res. 2021; 8121407.

Tomoko Koda, et al.

Enzyme-treated Asparagus Extract (ETAS) Enhances Memory in Normal Rats and Induces Neurite-outgrowth in PC12 Cells

Nat Prod Commun. 2017; 12(10): 1631-1633.

Takuya Sakurai, et al.

Enzyme-treated Asparagus officinalis Extract Shows Neuroprotective Effects and Attenuates Cognitive Impairment in Senescenceaccelerated Mice

Nat Prod Commun. 2014; 9(1): 101-6.

Kensaku Sugaya, et al.

Proline-3-alkyldiketopiperazine from asparagus (Asparaprolines)

Development of anti-fatigue, anti-stress, and sleep-improving foods. 2020: 209-217.

Hiroshi Nishioka

Development of functional food ETAS from Hokkaido asparagus and establishment of sales network

Journal of Agricultural and Food Technology. 2019; 14(4), 50-53.

Takahiro Maeda

ETAS®, a new functional food material with anti-stress properties

Journal of Agricultural and Food Technology. 2016; 12(2), 22-27.

Jun Takanari

New Functionality of Mood Food “Enzyme-Treated Asparagus Extract

FOOD STYLE 21. 2016; 20(6): 55-58.

Takahiro Maeda, et al.

A new anti-stress functional food ingredient “ETAS”, an enzyme-treated asparagus extract

New Food Industry. 2015; 57(1): 27-34.

Jun Takanari, et al.

Development of anti-stress food material “enzyme-treated asparagus extract”

FOOD STYLE 21. 2013; 17(8): 64-66.

Tomohiro Ito, et al.

Enhanced heat shock protein expression by enzyme-treated asparagus extract

New Food Industry. 2012; 54(8): 1-9.

Khoi Thieu Ho, et al.

Synergistic effect of standardized extract of Asparagus officinalis stem and heat shock on progesterone synthesis with lipid droplets and mitochondrial function in bovine granulosa cells

J Steroid Biochem Mol Biol. 2023; 225: 106181.

Khoi Thieu Ho, et al.

A standardized extract of Asparagus officinalis stem improves HSP70-mediated redox balance and cell functions in bovine cumulus-granulosa cells

Sci Rep. 2021; 11(1): 18175.

Ken Shirato, et al.

Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Molecules. 2021; 26(20): 6189.

Shoichiro Inoue, et al.

Isolation and Structure Determination of a Heat Shock Protein Inducer, Asparagus-derived Proline-containing 3-Alkyldiketopiperazines (Asparaprolines), from a standardized extract of Asparagus officinalis stem

Nat Prod Commun. 2020; 15(3): 1-7.

Tomohiro Ito, et al.

Isolation, structural elucidation, and biological evaluation of a 5-hydroxymethyl-2-furfural derivative, asfural, from enzyme-treated asparagus extract

J Agric Food Chem. 2013; 61(38): 9155-9.

Tomoko Koda, et al.

Effects of a Standardized Extract of Asparagus officinalis Stem on Photoaging in the Epidermal Layer of the Skin Using Cultured Keratinocytes

Nat Prod Commun. 2019; DOI: 10.1177/1934578X19857345.

Ken Shirato, et al.

ETAS®50 Attenuates Ultraviolet-B-Induced Interleukin-6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

Evid Based Complement Alternat Med. 2018; 2018: 1547120.

Ken Shirato, et al.

Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts

Evid Based Complement Alternat Med. 2018; 2018: 5072986.

Ken Shirato, et al.

A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study

Environ Health Prev Med. 2018; 23(1): 40.

Ken Shirato, et al.

Enzyme-Treated Asparagus Extract Prevents’Hydrogen Peroxide-Induced Pro-Inflammatory Responses by Suppressing p65 Nuclear Translocation in Skin L929 Fibroblasts

Nat Prod Commun. 2016; 11(12): 1883-1888.

Ken Shirato, et al.

Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells

Nat Prod Commun. 2016; 11(5): 677-80.

Francesca Paganelli, et al.

The Combination of AHCC and ETAS Decreases Migration of Colorectal Cancer Cells, and Reduces the Expression of LGR5 and Notch1 Genes in Cancer Stem Cells: A Novel Potential Approach for Integrative Medicine

Pharmaceuticals. 2021; 14(12): 1325.

Yasuhiro Kuramitsu, et al.

Enzyme-treated Asparagus Extract Down-regulates Heat Shock Protein 27 of Pancreatic Cancer Cells

In Vivo. 2018; 32(4): 759-763.

Asuka Yasueda, et al.

Safety Evaluation of a Standardised Extract of Asparagus officinalis Stem in Healthy Volunteers: a Double-Blind and Randomised Controlled Trial

Journal of Herbal Medicine. 2023; 42:100789

Tomohiro Ito, et al.

Toxicological assessment of enzyme-treated asparagus extract in rat acute and subchronic oral toxicity studies and genotoxicity tests

Regul Toxicol Pharmacol. 2014; 68(2): 240-9.